Basic Microscope Kit
activity:basic-microscope

The Basic Microscope Kit is an introductory Do-It-Yourself microscope made from affordable and easy to find parts. It's based on [the Hackteria microscope](https://publiclab.org/notes/partsandcrafts/10-26-2017/making-a-hackteria-microscope), the OpenFlexure Microscope, and @maxliboiron's work at http://CivicLaboratory.nl ( #13503 ), among other sources. (above image by @partsandcrafts) ## Goals This project was started as a collaboration between Public Lab and [Parts & Crafts](http://partsandcrafts.org). The goals of the project are: 1. offer a very easy starting point for DIY microscopy 2. act as a stepping-stone to more advanced DIY microscopes 3. be as affordable and simple as possible while still providing great microscopic images 4. be good enough to view 2-5 micron size particles (see [sample images below](#Sample+images)) ##About the parts ### General parts list * a lens (objective) * a [Raspberry Pi](/tag/raspberry-pi) * a [Raspberry Pi camera](/pi-camera) * a light source * a rigid stage to connect these all together ###Example parts list for building a Basic Microscope: * [Raspberry Pi camera](https://www.amazon.com/SainSmart-Camera-Module-Webcam-Raspberry/dp/B00FGKYHXA/ref=sr_1_1_sspa?s=electronics&ie=UTF8&qid=1516378086&sr=1-1-spons&keywords=raspberry+pi+camera&psc=1) * [Raspberry Pi Zero W](https://www.amazon.com/CanaKit-Raspberry-Wireless-Starter-Official/dp/B06XJQV162/) * [a micro SD card](https://www.amazon.com/SanDisk-MicroSDHC-SDSDQM-B35A-Without-Adapter/dp/B004ZIENBA/ref=sr_1_4?ie=UTF8) * [a USB light](https://www.amazon.com/I-MU-Flexible-Gooseneck-Brightness-Gray-White/dp/B071Y3P8ZC/) * [a lens (objective x40)](http://www.amscope.com/40x-achromatic-microscope-objective.html) * [a hackteria microscope stage](https://publiclab.org/notes/partsandcrafts/01-22-2018/building-the-microscope-stage) (with modifications from Parts and Crafts!) * [a 3D printed objective adapter](https://github.com/rwb27/openflexure_microscope/blob/master/stl/optics_picam2_rms.stl) * the bolts to hold it together Extras to help you get started: * [Microscope slides](https://www.amazon.com/gp/product/B008JFRP1K/ref=od_aui_detailpages00?ie=UTF8&psc=1) * [A microscope stage calibration slide](http://www.amscope.com/microscope-stage-calibration-slide-for-usb-camera-0-01mm-stage-micrometer.html) * [Microscope Immersion Oil](https://www.amazon.com/gp/product/B007PD4IZS/ref=ox_sc_act_title_1?smid=A35BV4NHHC5J5C&psc=1) * Rubberbands * A magnet **** ## Getting started Start with the [Overview activity, here](/notes/partsandcrafts/11-26-2017/building-a-raspberry-pi-microscope). Then see [the activities below](#Activities) for each step in detail. _Basic Prototyping Kit now available for sale in the [Public Lab store](https://publiclab.myshopify.com/collections/under-development/products/basic-microscope-prototyping-kit-preorder?variant=7861722578972)!_ **** ## Questions [questions:basic-microscope] ![](https://publiclab.org/system/images/photos/000/022/864/medium/Screenshot_2017-12-08_at_5.12.19_PM.png) ## Activities [activities:basic-microscope] ## Challenges We're working to refine and improve DIY spectrometry on a number of fronts; here, take a look at the leading challenges we're hoping to solve, and post your own. For now, we're using the Q&A feature, so just click "Ask a question" to post your own challenge. [questions:basic-microscope-challenge] **** ## People Folks involved in getting this project started include: [people:group:partsandcrafts] **** ## Sample images [![](/system/images/photos/000/022/598/large/sample_1.jpg)](/system/images/photos/000/022/598/original/sample_1.jpg) [![](/system/images/photos/000/022/597/large/sample_3.jpg)](/system/images/photos/000/022/597/original/sample_3.jpg) ...


Author Comment Last activity Moderation
georgegleed1 "Hi, this looks like a great project and I'd love to try it out. The 3D file links appear to be dead and produce a database error. Any chance anyone..." | Read more » 11 months ago
fongvania "I did this! " | Read more » 12 months ago
eureka_chen "Getting someone else to cut the material on behalf of the laser is not costly if it is only once or twice. But once the number of times, then it is..." | Read more » 12 months ago
tyagiarti22 "Hi, great project! I have a question, not exactly related to the raspberry pi microscope but I'm hoping you'd be able to help me out... I'm using a..." | Read more » about 1 year ago
nashdracones "Correct on the Cardboard Lens, I suspected the same after finding it very hard and expensive to get a openflexure tube lens. OpenLab has a scope t..." | Read more » over 1 year ago
vishal_jv1511 "awesome articles Please visit also -> Digital Marketing Institute in Jaipur seo training in jaipur " | Read more » over 2 years ago
tapariayogesh "What is the viewing angle of the camera lens? " | Read more » over 2 years ago
warren "Obviously we love to see any improved 3d printed parts and would love to add to the design. Just so people know, we try to print these to be solid,..." | Read more » almost 3 years ago
warren "Sadie, just curious, how does your adhere to the board? I love the little grippy bits that hold the lens itself! " | Read more » almost 3 years ago
warren "Or try https://publiclab.github.io/infragram/ for a more up-to-date version of that webapp! " | Read more » almost 3 years ago
KathleenRobinson "wao good to see these type of spy camera " | Read more » about 3 years ago
warren "Oh awesome! " | Read more » about 3 years ago
SadiePrego "Hey no problem! I actually made one myself which works pretty well - no tape needed, here's the file: Webcam_lens_adapter_v2.stl " | Read more » about 3 years ago
warren "Oh sorry! I misunderstood! Here, i've added that too now, but note that I believe I just found a standard m12 on thingiverse and resized it. So the..." | Read more » about 3 years ago
SadiePrego "Hey @warren the .stl file doesn't look like the 3D printed adapter in ur picture (screenshot below), it looks like it might be the adapter for the ..." | Read more » about 3 years ago
SadiePrego "thank u @warren! Going to try it out, unfortunately are makerbot doesnt take gcode which is a lil surprising but I guess cause its a newer model " | Read more » about 3 years ago
delhinsim "It's wow! https://www.nsim.in/digital-marketing-course-in-dubai/ " | Read more » about 3 years ago
warren "It's this one! https://github.com/publiclab/community-microscope/blob/master/lens-adapter.stl " | Read more » about 3 years ago
warren "Oh gosh, well it's not super nice, and i think we may literally just have crudely stretched them to make gcode, but, the original is here: https://..." | Read more » about 3 years ago
SadiePrego "@bronwen @warren is there any chance these files exist as STL? Thanks! " | Read more » about 3 years ago
jenjimah " Hi Warren, Sure I'd be happy to try to add that in, it's only 2 files that need to be updated after all. However before that, there are a few thin..." | Read more » about 3 years ago
warren " This is great! Indeed, this is a good approach, although I found that not all computers could use an internet gadget either without drivers, it's ..." | Read more » about 3 years ago
jenjimah "I did this! Hardware: Raspberry Pi Zero W Software: Raspbian Stretch Lite, Windows 10 (on PC) Connection: USB configured as ethernet device; SSH i..." | Read more » about 3 years ago
jenjimah " Thanks for the link! I also wasn't able to use the wifi method of connecting so I also connected over usb. I had to follow the whole tutorial from..." | Read more » about 3 years ago